Electronic supplementary material


Download 13.42 Kb.
NameElectronic supplementary material
A typeDocumentation
manual-guide.com > manual > Documentation
Electronic supplementary material
ESM Table 2 MIAME compliance information for Affymetrix experiments


MIAME guideline

Description

Documentation

Sample description







  Labelling protocol

BioArray High-Yield RNA Transcript Labeling kit

http://www.affymetrix.com/support/technical/manual/expression_manual.affx

  Hybridisation protocol

URL to Affymetrix

http://www.affymetrix.com/support/technical/manual/expression_manual.affx

  QC–Starting RNA

Agilent 2100 Bioanalyzer Electropherogram

See Hao et al. (2006)a

  QC-Test3 array

GAPDH 3′:5′ ratio

Ratios <3.0




β-Actin 3′:5′ value

Ratios <3.0




BIOB

Present




BIOC

Present




BIOD

Present




CREX

Present




Are the values ascending?

Ascending order

Data







  Generation

Hardware

Affymetrix Hybridisation Oven 640

Affymetrix Fluidics Station 400

Affymetrix GeneChip Scanner 3000

  Image analysis software




GCOS 1.2 – absolute analysis

https://www.affymetrix.com/support/file_download.affx?onloadforward=/support/downloads/manuals/gcos_manual.zip (GCOS User’s Guide, Version 1.2)

User Defined Target Value: 150

All probes normalisation: 1

  Normalisation QC

Scaling factors

Within threefold of each other




Per cent of genes called present

Similar

  Background and Q

Average background

<100.0




Raw Q

Similar

  Image visual inspection

Bubbles/scratches/other

NOT present




Grid corners aligned

YES

Array design







  Name of array

HG-U133A




  Description

URL to the HGU133A array

http://www.affymetrix.com/products/arrays/specific/hgu133.affx

  Description

URL to the HGU133A array

http://www.affymetrix.com/products/arrays/specific/hgu133.affx

aBrands K, Colvin E, Williams LJ, Wang R, Lock RB, Tuch BE (2008) Reduced immunogenicity of first-trimester human fetal pancreas. Diabetes 57:627–634

Share in:

Related:

Electronic supplementary material iconElectronic Supplementary Material

Electronic supplementary material iconElectronic Supplementary Material

Electronic supplementary material iconSupplementary material

Electronic supplementary material iconSupplementary 1: Material and methods

Electronic supplementary material iconSupplementary Online Material

Electronic supplementary material iconElectronic Supplementary Information

Electronic supplementary material iconSupplementary Material a,b,g -trifluoro alkanes: a stereoselective...

Electronic supplementary material iconSupplementary material for: Spirolactone syntheses through a rhodium-catalyzed...

Electronic supplementary material iconSupplementary material a physiologically Based Pharmacokinetic Model...

Electronic supplementary material iconThis online resource list is organised under material suitable for...

Electronic supplementary material icon1991 This material is the property of The Lutheran Church—Missouri...

Electronic supplementary material iconSupplementary Materials and Methods and Supplementary Tables

Electronic supplementary material iconReaders Note: Original material is in italics and surrounded by quotation...

Electronic supplementary material iconElectronic Media Electronic Story Archive 1994 to

Electronic supplementary material icon21 cfr part 11; Electronic Records; Electronic Signatures

Electronic supplementary material iconElectronic Warfare Operator Operated overland and ocean radar, infrared...

Electronic supplementary material iconUser Manual for electronic upload of vsat activation / Surrender...

Electronic supplementary material iconSub.: Procedure for electronic filing of Central Excise and Service...

Electronic supplementary material iconSupplementary Information

Electronic supplementary material iconSupplementary information




manual


When copying material provide a link © 2017
contacts
manual-guide.com
search